1. Cholinga: Ndondomekoyi ikufuna kupereka ndondomeko yokhazikika ya maopaleshoni a aseptic ndi kuteteza zipinda zosabala.
2. Kuchuluka kwa ntchito: labotale yoyezetsa zamoyo
3. Munthu Wodalirika: QC Supervisor Tester
4.Tanthauzo: Palibe
5. Njira zodzitetezera
Kuchita mosamalitsa maopaleshoni aaseptic kuti mupewe kuipitsidwa ndi tizilombo;Ogwiritsa ntchito ayenera kuzimitsa nyali ya UV asanalowe m'chipinda chosabala.
6.Njira
6.1.Chipinda chosabala chimayenera kukhala ndi chipinda chopangira opaleshoni komanso chipinda chosungiramo zinthu.Chiyero cha chipinda chopangira opaleshoni chiyenera kufika m'kalasi 10000. Kutentha kwa m'nyumba kuyenera kusungidwa pa 20-24 ° C ndipo chinyezi chiyenera kusungidwa pa 45-60%.Ukhondo wa benchi yoyera uyenera kufika m'kalasi la 100.
6.2.Chipinda chosabala chiyenera kukhala chaukhondo, ndipo sikuloledwa kuunjikira zinyalala kuti zisaipitsidwe.
6.3.Kuletsa kuipitsidwa kwa zida zonse zotsekera ndi media media.Amene ali ndi kachilombo ayenera kusiya kuwagwiritsa ntchito.
6.4.Chipinda chosabala chiyenera kukhala ndi mankhwala ophera tizilombo togwira ntchito, monga 5% cresol solution, 70% mowa, 0.1% chlormethionine solution, etc.
6.5.Chipinda chopanda chiberekero chiyenera kukhala chosawilitsidwa ndikuyeretsedwa ndi mankhwala oyenera ophera tizilombo pofuna kuwonetsetsa kuti ukhondo wa mchipindacho ukukwaniritsa zofunikira.
6.6.Zida zonse, zida, mbale ndi zinthu zina zomwe ziyenera kubweretsedwa m'chipinda chosabala zikuyenera kukulungidwa molimba ndi kutsekedwa ndi njira zoyenera.
6.7.Asanalowe m'chipinda chosabala, ogwira ntchito ayenera kusamba m'manja ndi sopo kapena mankhwala ophera tizilombo, ndiyeno nkusintha zovala zapadera zantchito, nsapato, zipewa, masks ndi magolovesi m'chipinda chokhalamo (kapena kupukutanso manja awo ndi 70% ethanol) asanalowe m'chipinda chopanda kanthu.Chitani ntchito mu chipinda cha bakiteriya.
6.8.Musanagwiritse ntchito chipinda chosabala, nyali ya ultraviolet m'chipinda chosabala iyenera kuyatsidwa kuti muyatse ndi kuthirira kwa mphindi 30, ndipo benchi yoyera iyenera kuyatsidwa kuti iwumbe mpweya nthawi yomweyo.Opaleshoniyo ikamalizidwa, chipinda chosabala chimayenera kutsukidwa munthawi yake ndikuyeretsedwa ndi kuwala kwa ultraviolet kwa mphindi 20.
6.9.Asanaunike, zotengera zakunja zoyeserera ziyenera kusungidwa bwino ndipo zisatsegulidwe kuti zipewe kuipitsidwa.Musanayang'ane, gwiritsani ntchito mipira 70% ya thonje ya mowa kuti muphe kunja.
6.10.Pa opaleshoni iliyonse, kuwongolera koyipa kuyenera kuchitidwa kuti muwone kudalirika kwa opaleshoni ya aseptic.
6.11.Mukayamwa madzi a bakiteriya, muyenera kugwiritsa ntchito mpira woyamwa kuti muyamwe.Osakhudza udzuwo ndi pakamwa pako.
6.12.Singano yoyikiramo iyenera kutsekedwa ndi moto musanagwiritse ntchito komanso mukatha.Pambuyo kuzirala, chikhalidwe akhoza inoculated.
6.13.Udzu, machubu oyesera, mbale za petri ndi ziwiya zina zomwe zimakhala ndi madzi a bakiteriya ziyenera kuviikidwa mu chidebe chotseketsa chomwe chili ndi 5% ya mankhwala ophera tizilombo toyambitsa matenda a Lysol, ndikuchotsedwa ndi kutsukidwa pakatha maola 24.
6.14.Ngati pali madzi a bakiteriya otayikira patebulo kapena pansi, muyenera kuthira nthawi yomweyo 5% carbolic acid solution kapena 3% Lysol pamalo okhudzidwa kwa mphindi zosachepera 30 musanawachiritse.Zovala zantchito ndi zipewa zikayipitsidwa ndi madzi a bakiteriya, ziyenera kuchotsedwa nthawi yomweyo ndikutsukidwa pambuyo pa kutsekeka kwa nthunzi.
6.15.Zinthu zonse zomwe zili ndi mabakiteriya amoyo ziyenera kupha tizilombo tisanatsukidwe pansi pa mpopi.Ndizoletsedwa kwambiri kuipitsa ngalande.
6.16.Chiwerengero cha anthu okhala m'zipinda zosabala chiyenera kuwonedwa mwezi uliwonse.Ndi benchi yoyera yotseguka, tengani mbale zingapo zosabala zokhala ndi mainchesi 90 mm, ndi kubaya 15 ml ya michere ya agar culture medium yomwe yasungunuka ndikuzizidwa mpaka pafupifupi 45 ° C.Mukatha kulimba, ikani mozondoka pa 30 mpaka 35 Ikani kwa maola 48 mu chofungatira cha ℃.Mukatsimikizira kusabereka, tengani mbale zitatu mpaka 5 ndikuziyika kumanzere, pakati ndi kumanja kwa malo ogwirira ntchito.Mukatsegula chivundikirocho ndi kuwavumbulutsa kwa mphindi 30, ikani mozondoka mu chofungatira cha 30 mpaka 35°C kwa maola 48 ndikuwatulutsa.fufuzani.Pafupifupi chiwerengero cha mabakiteriya osiyanasiyana pa mbale mu kalasi 100 malo oyera sayenera upambana 1 koloni, ndipo pafupifupi chiwerengero mu kalasi 10000 woyera chipinda si upambana 3 ankawalamulira.Ngati malire apyola, chipinda chosabalacho chiyenera kutetezedwa ndi tizilombo toyambitsa matenda mpaka kuyendera mobwerezabwereza kukwaniritse zofunikira.
7. Onani mutuwo (Njira Yoyang'anira Kusabereka) mu "Njira Zoyang'anira Mankhwala Osokoneza Bongo" ndi "China Standard Operating Practices for Drug Inspection".
8. Dipatimenti Yogawa: Dipatimenti Yoyang'anira Ubwino
Chitsogozo chaukadaulo chazipinda:
Titapeza malo owuma komanso zinthu zosabala, tiyenera kukhalabe ndi moyo wosabala kuti tiphunzire za tizilombo todziwika bwino kapena kugwiritsa ntchito ntchito zake.Kupanda kutero, tizilombo tating'onoting'ono tosiyanasiyana tochokera kunja titha kusakanikirana mosavuta. Chodabwitsa cha kusakanikirana kwa tizilombo tosafunikira kuchokera kunja kumatchedwa kuti mabakiteriya owononga mu microbiology.Kupewa kuipitsidwa ndi njira yofunika kwambiri pantchito ya microbiological.Kutsekereza kwathunthu mbali imodzi ndikupewa kuipitsidwa mbali inayo ndi mbali ziwiri za njira ya aseptic.Komanso, tiyenera kupewa tizilombo tomwe tikuphunzira, makamaka tizilombo toyambitsa matenda kapena tizilombo tating'onoting'ono tomwe tilibe m'chilengedwe, kuti tisathawe muzitsulo zathu zoyesera kupita ku chilengedwe chakunja.Pazifukwa izi, mu microbiology, pali njira zambiri.
Chipinda chosabala nthawi zambiri chimakhala kachipinda kakang'ono kokhazikitsidwa mu labotale ya microbiology.Ikhoza kumangidwa ndi mapepala ndi galasi.Dera liyenera kukhala lalikulu kwambiri, pafupifupi 4-5 lalikulu mita, ndipo kutalika kwake kuyenera kukhala pafupifupi 2.5 metres.Chipinda chosungiramo chitetezo chiyenera kukhazikitsidwa kunja kwa chipinda chosabala.Chitseko cha chipinda chotchinga ndi chitseko cha chipinda chosabala zisayang'ane mbali imodzi kuletsa kutuluka kwa mpweya kubweretsa mabakiteriya osiyanasiyana.Zipinda zonse ziwiri zosakhala ndi buffer ziyenera kukhala zopanda mpweya.Zida zolowera m'nyumba ziyenera kukhala ndi zida zosefera mpweya.Pansi ndi makoma a chipinda chosabalacho chiyenera kukhala chosalala, chovuta kusunga dothi komanso chosavuta kuyeretsa.Malo ogwirira ntchito ayenera kukhala amtundu.Zipinda zonse zosabala ndi zotchingira zili ndi nyali za ultraviolet.Nyali za ultraviolet m'chipinda chosabala ndi mita imodzi kuchokera pamalo ogwirira ntchito.Ogwira ntchito omwe alowa m'chipinda chosabala ayenera kuvala zovala zosabala ndi zipewa.
Pakadali pano, zipinda zosabala zambiri zimapezeka m'mafakitole a microbiology, pomwe ma laboratories ambiri amagwiritsa ntchito benchi yoyera.Ntchito yayikulu ya benchi yoyera ndikugwiritsa ntchito chipangizo chalaminar mpweya wotuluka kuchotsa fumbi ting'onoting'ono tosiyanasiyana kuphatikiza tizilombo tating'onoting'ono pantchito.Chipangizo chamagetsi chimalola kuti mpweya udutse fyuluta ya hepa ndikulowa pamwamba pa ntchito, kotero kuti malo ogwirira ntchito nthawi zonse amayang'aniridwa ndi mpweya wosabala.Komanso, pali chinsalu chotchinga cham'mwamba chothamanga kwambiri kumbali yomwe ili pafupi ndi kunja kuti muteteze mpweya wa bakiteriya wakunja kulowa.
M'malo ovuta, mabokosi amatabwa atha kugwiritsidwanso ntchito m'malo mwa benchi yoyera.Bokosi losabala lili ndi kapangidwe kosavuta komanso kosavuta kusuntha.Pali mabowo awiri kutsogolo kwa bokosi, omwe amatsekedwa ndi zitseko zokoka-koka pamene sizikugwira ntchito.Mukhoza kuwonjezera manja anu mkati mwa ntchito.Kumtunda kwa kutsogolo kuli ndi galasi kuti azitha kugwira ntchito mkati.Mkati mwa bokosi muli nyali ya ultraviolet, ndipo ziwiya ndi mabakiteriya amatha kuikidwa kudzera pakhomo laling'ono pambali.
Njira zogwirira ntchito za Aseptic pakadali pano sizimangotenga gawo lofunikira kwambiri pakufufuza ndi kagwiritsidwe ntchito ka tizilombo toyambitsa matenda, komanso zimagwiritsidwanso ntchito kwambiri muzachilengedwe zambiri.Mwachitsanzo, ukadaulo wa transgenic, ukadaulo wa monoclonal antibody, ndi zina zambiri.
Nthawi yotumiza: Mar-06-2024