1. Cholinga: Njirayi ikufuna kupereka njira yokhazikika yogwiritsira ntchito zinthu zopanda poizoni komanso kuteteza zipinda zopanda poizoni.
2. Kuchuluka kwa ntchito: labotale yoyesera zamoyo
3. Munthu Wodalirika: Woyesa Woyang'anira QC
4. Tanthauzo: Palibe
5. Njira zodzitetezera
Chitani ntchito zoteteza ku mabakiteriya kuti mupewe kuipitsidwa ndi tizilombo toyambitsa matenda; ogwira ntchito ayenera kuzimitsa nyali ya UV asanalowe m'chipinda chopanda tizilombo toyambitsa matenda.
6. Njira
6.1. Chipinda choyeretsera chiyenera kukhala ndi chipinda choyeretsera choyeretsera komanso chipinda chosungiramo zinthu. Ukhondo wa chipinda choyeretsera choyeretsera uyenera kufika pa kalasi 10000. Kutentha kwa mkati kuyenera kukhala pa 20-24°C ndipo chinyezi chiyenera kukhala pa 45-60%. Ukhondo wa benchi yoyera uyenera kufika pa kalasi 100.
6.2. Chipinda chopanda tizilombo toyambitsa matenda chiyenera kukhala choyera, ndipo n'koletsedwa kwambiri kusonkhanitsa zinyalala kuti tipewe kuipitsidwa.
6.3. Pewani kuipitsidwa ndi zida zonse zoyeretsera ndi zofalitsa zofalitsa nkhani. Zomwe zaipitsidwa ziyenera kusiya kuzigwiritsa ntchito.
6.4. Chipinda choyeretsera chiyenera kukhala ndi mankhwala ophera tizilombo toyambitsa matenda, monga yankho la 5% cresol, 70% alcohol, yankho la 0.1% chlormethionine, ndi zina zotero.
6.5. Chipinda choyeretsedwera chiyenera kutsukidwa nthawi zonse ndi kutsukidwa ndi mankhwala ophera tizilombo toyambitsa matenda kuti chikhale choyera bwino.
6.6. Zida zonse, zida, mbale ndi zinthu zina zomwe ziyenera kubweretsedwa m'chipinda chopanda tizilombo ziyenera kukulungidwa bwino ndikutsukidwa ndi njira zoyenera.
6.7. Asanalowe m'chipinda chopanda tizilombo toyambitsa matenda, ogwira ntchito ayenera kusamba m'manja ndi sopo kapena mankhwala ophera tizilombo toyambitsa matenda, kenako n'kuvala zovala zapadera zogwirira ntchito, nsapato, zipewa, zophimba nkhope ndi magolovesi m'chipinda chosungiramo mankhwala (kapena kupukuta manja awo ndi 70% ethanol) asanalowe m'chipinda chopanda tizilombo toyambitsa matenda. Chitani opaleshoni m'chipinda chopanda tizilombo toyambitsa matenda.
6.8. Musanagwiritse ntchito chipinda chopanda tizilombo toyambitsa matenda, nyali ya ultraviolet m'chipinda chopanda tizilombo toyambitsa matenda iyenera kuyatsidwa kuti ipereke kuwala ndi kuyeretsa kwa mphindi zoposa 30, ndipo benchi yoyera iyenera kuyatsidwa kuti ipumule mpweya nthawi yomweyo. Ntchito ikatha, chipinda chopanda tizilombo toyambitsa matenda chiyenera kutsukidwa nthawi yake kenako nkutsukidwa ndi kuwala kwa ultraviolet kwa mphindi 20.
6.9. Musanayang'ane, phukusi lakunja la chitsanzo choyesera liyenera kusungidwa bwino ndipo lisatsegulidwe kuti lisaipitse. Musanayang'ane, gwiritsani ntchito mipira ya thonje ya 70% ya mowa kuti muphe tizilombo toyambitsa matenda pamwamba pakunja.
6.10. Pa opaleshoni iliyonse, kuwunika koyipa kuyenera kuchitika kuti muwone ngati ntchito ya aseptic ndi yodalirika.
6.11. Mukayamwa madzi a bakiteriya, muyenera kugwiritsa ntchito mpira woyamwa kuti muyamwe. Musakhudze udzuwo mwachindunji ndi pakamwa panu.
6.12. Singano yopangira katemera iyenera kutsukidwa ndi moto musanagwiritse ntchito komanso mutagwiritsa ntchito. Pambuyo poziziritsa, mbewuyo imatha kutsukidwa.
6.13. Ma udzu, machubu oyesera, mbale za petri ndi ziwiya zina zokhala ndi madzi a bakiteriya ziyenera kuviikidwa mu chidebe choyeretsera chomwe chili ndi 5% ya yankho la Lysol kuti liphere matenda, ndikuchotsedwa ndikutsukidwa patatha maola 24.
6.14. Ngati pali madzi a bakiteriya omwe atayika patebulo kapena pansi, muyenera kuthira nthawi yomweyo 5% ya carbon acid kapena 3% ya Lysol pamalo oipitsidwawo kwa mphindi zosachepera 30 musanawachiritse. Ngati zovala zantchito ndi zipewa zaipitsidwa ndi madzi a bakiteriya, ziyenera kuchotsedwa nthawi yomweyo ndikutsukidwa mutaziyeretsa ndi nthunzi yothamanga kwambiri.
6.15. Zinthu zonse zomwe zili ndi mabakiteriya amoyo ziyenera kutsukidwa ndi mankhwala ophera tizilombo toyambitsa matenda musanatsukidwe pansi pa mpopi. N'koletsedwa kwambiri kuipitsa chimbudzi.
6.16. Chiwerengero cha makoloni m'chipinda chopanda tizilombo chiyenera kufufuzidwa mwezi uliwonse. Benchi yoyera ikatsegulidwa, tengani mbale zingapo zopanda tizilombo zomwe zili ndi mainchesi amkati a 90 mm, ndikulowetsamo mankhwala ophera tizilombo pafupifupi 15 ml ya michere yopangidwa ndi agar yomwe yasungunuka ndikuziziritsidwa kufika pa 45°C. Mukayiyika mozondoka, ikani mozondoka pa 30 mpaka 35 Incubate kwa maola 48 mu ℃ incubator. Mukatsimikizira kuti ndi yopanda tizilombo, tengani mbale 3 mpaka 5 ndikuziyika kumanzere, pakati ndi kumanja kwa malo ogwirira ntchito. Mukatsegula chivundikirocho ndikuchiyika pamalo ogwirira ntchito kwa mphindi 30, ziyikeni mozondoka mu 30 mpaka 35°C incubator kwa maola 48 ndikuzitulutsa. Yang'anani. Chiwerengero cha mabakiteriya osiyanasiyana pa mbale m'dera loyera la kalasi 100 sichiyenera kupitirira koloni imodzi, ndipo chiwerengero chapakati m'chipinda choyera cha kalasi 10000 sichiyenera kupitirira makoloni atatu. Ngati malire apitirira, chipinda chopanda tizilombo toyambitsa matenda chiyenera kutsukidwa bwino mpaka kuwunika mobwerezabwereza kukwaniritse zofunikira.
7. Onani mutu (Njira Yowunikira Matenda Osabereka) mu "Njira Zowunikira Mankhwala Osawononga" ndi "Machitidwe Oyendetsera Ntchito Okhazikika ku China Oyang'anira Mankhwala Osokoneza Bongo".
8. Dipatimenti Yogawa: Dipatimenti Yoyang'anira Ubwino
Malangizo aukadaulo oyeretsa chipinda:
Tikapeza malo osabala komanso zinthu zosabala, tiyenera kukhalabe ndi malo osabala kuti tiphunzire tizilombo todziwika bwino kapena kugwiritsa ntchito ntchito zawo. Kupanda kutero, tizilombo toyambitsa matenda osiyanasiyana ochokera kunja tingasakanikirane mosavuta. Chochitika cha kusakaniza tizilombo toyambitsa matenda tosafunikira kuchokera kunja chimatchedwa kuipitsa mabakiteriya mu microbiology. Kupewa kuipitsidwa ndi njira yofunika kwambiri pa ntchito ya microbiology. Kuipitsa kwathunthu mbali imodzi ndi kupewa kuipitsidwa mbali inayi ndi mbali ziwiri za njira yopewera kuipitsidwa. Kuphatikiza apo, tiyenera kuletsa tizilombo toyambitsa matenda zomwe tikuziphunzira, makamaka tizilombo toyambitsa matenda kapena tizilombo toyambitsa matenda tomwe sitili m'chilengedwe, kuti tisatuluke m'ziwiya zathu zoyesera kupita kumalo akunja. Pazifukwa izi, mu microbiology, pali njira zambiri.
Chipinda chopanda tizilombo nthawi zambiri chimakhala chipinda chaching'ono chomwe chimayikidwa mu labotale ya microbiology. Chingamangidwe ndi mapepala ndi galasi. Malo ake sayenera kukhala akulu kwambiri, pafupifupi mamita 4-5, ndipo kutalika kwake kuyenera kukhala pafupifupi mamita 2.5. Chipinda chosungira mpweya chiyenera kuyikidwa kunja kwa chipinda chopanda tizilombo. Chitseko cha chipinda chosungira mpweya ndi chitseko cha chipinda chopanda tizilombo siziyenera kuyang'ana mbali imodzi kuti mpweya usalowe m'malo osiyanasiyana. Chipinda chopanda tizilombo ndi chipinda chosungira mpweya ziyenera kukhala zopanda mpweya. Zipangizo zopumira mpweya zamkati ziyenera kukhala ndi zipangizo zosefera mpweya. Pansi ndi makoma a chipinda chopanda tizilombo ziyenera kukhala zosalala, zovuta kusunga dothi komanso zosavuta kuyeretsa. Malo ogwirira ntchito ayenera kukhala ofanana. Chipinda chopanda tizilombo ndi chipinda chosungira mpweya chili ndi magetsi a ultraviolet. Magetsi a ultraviolet m'chipinda chopanda tizilombo ali pamtunda wa mita imodzi kuchokera pamalo ogwirira ntchito. Ogwira ntchito omwe amalowa m'chipinda chopanda tizilombo ayenera kuvala zovala zoyeretsera mpweya ndi zipewa.
Pakadali pano, zipinda zoyera zimapezeka kwambiri m'mafakitale a sayansi ya tizilombo toyambitsa matenda, pomwe ma labotale ambiri amagwiritsa ntchito benchi yoyera. Ntchito yayikulu ya benchi yoyera ndikugwiritsa ntchito chipangizo choyeretsera mpweya kuti muchotse fumbi laling'ono kuphatikizapo tizilombo toyambitsa matenda pamalo ogwirira ntchito. Chipangizo chamagetsi chimalola mpweya kudutsa mu fyuluta ya hepa kenako kulowa pamalo ogwirira ntchito, kotero kuti malo ogwirira ntchito nthawi zonse azikhala pansi pa ulamuliro wa mpweya woyera. Kuphatikiza apo, pali nsalu yotchinga mpweya yothamanga kwambiri kumbali yomwe ili pafupi ndi kunja kuti mpweya wa bakiteriya usalowe.
M'malo ovuta, mabokosi opangidwa ndi matabwa opangidwa ndi matabwa angagwiritsidwenso ntchito m'malo mwa benchi yoyera. Bokosi lopangidwa ndi matabwa lili ndi kapangidwe kosavuta ndipo ndi losavuta kusuntha. Pali mabowo awiri kutsogolo kwa bokosi, omwe amatsekedwa ndi zitseko zokoka pamene sizikugwira ntchito. Mutha kutambasula manja anu mkati mukamagwiritsa ntchito. Gawo lakumtunda la kutsogolo lili ndi galasi kuti lithandize kugwira ntchito mkati. Pali nyali ya ultraviolet mkati mwa bokosilo, ndipo ziwiya ndi mabakiteriya zimatha kulowetsedwa kudzera pakhomo laling'ono m'mbali.
Njira zogwiritsira ntchito mankhwala osokoneza bongo pakadali pano sizimangogwira ntchito yofunika kwambiri pa kafukufuku wa tizilombo toyambitsa matenda komanso kugwiritsa ntchito tizilombo toyambitsa matenda, komanso zimagwiritsidwanso ntchito kwambiri mu sayansi zambiri za biotechnology. Mwachitsanzo, ukadaulo wa transgenic, ukadaulo wa monoclonal antibody, ndi zina zotero.
Nthawi yotumizira: Marichi-06-2024